Characterization of Yeast Strains with Ketoreductase Activity for Bioreduction of Ketones

Authors

  • Pál Csuka
    Affiliation
    Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rkp. 3, Hungary
  • László Nagy-Győr
    Affiliation
    Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rkp. 3, Hungary
  • Zsófia Molnár
    Affiliation
    Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rkp. 3, Hungary
    Institute of Enzymology, ELKH Research Center of Natural Sciences, Hungarian Academy of Sciences (HAS), H-1117 Budapest, Magyar tudósok krt. 2, Hungary

  • Csaba Paizs
    Affiliation
    Biocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, RO-400028 Cluj-Napoca, Arany János str. 11, Romania
  • Viktória Bódai
    Affiliation
    Fermentia Microbiological Ltd, H-1049 Budapest, Berlini út 47-49, Hungary
  • László Poppe
    Affiliation
    Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, H-1111 Budapest, Műegyetem rkp. 3, Hungary
    Biocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, RO-400028 Cluj-Napoca, Arany János str. 11, Romania
    SynBiocat LLC, H-1172 Budapest, Szilasliget u. 3., Hungary

https://doi.org/10.3311/PPch.17429

Abstract

This study describes six yeast strains for stereoselective ketone reductions. The reaction conditions for the yeast strains (Pichia carsonii, Lodderomyces elongisporus, Candida norvegica, Candida guillermondi, Debaromyces fabryi and Candida parapsilosis) were optimized in a design of experiments for three ketones of different properties. The pH tolerance, temperature stability and productivity of the bioreductions with lyophilized cells of the yeast strains were characterized. In several cases, the optimized bioreductions resulted in enantiopure alcohols (ee > 99 %) with conversions ranging from moderate to excellent.

Keywords:

ketoreductase, yeast, whole-cell bioreduction, cofactor regeneration, Design of Experiments (DOE)

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Published Online

2021-05-17

How to Cite

Csuka, P., Nagy-Győr, L., Molnár, Z., Paizs, C., Bódai, V., Poppe, L. “Characterization of Yeast Strains with Ketoreductase Activity for Bioreduction of Ketones”, Periodica Polytechnica Chemical Engineering, 65(3), pp. 299–307, 2021. https://doi.org/10.3311/PPch.17429

Issue

Vol. 65 No. 3 (2021)

Section

Articles